Shimaoka Tsuyoshi, Maezono Hazuki, Ono Shunka, Asahi Yoko, Kawanishi Yuzo, Klanliang Kittipit, Takahashi Yusuke, Hayashi Mikako
Department of Restorative Dentistry and Endodontology, Graduate School of Dentistry, The University of Osaka, 1-8, Yamadaoka, Suita, Osaka, 565-0871, Japan.
Department of Restorative Dentistry and Periodontology, Division of Endodontics, Chiang Mai University, Chiang Mai, Thailand.
Sci Rep. 2025 Oct 16;15(1):36215. doi: 10.1038/s41598-025-20131-5.
Oral biofilms contribute to apical periodontitis, a major cause of tooth loss. This study evaluated the potential of an on-demand aqueous chlorine dioxide solution, "matching transformation system" (MA-T), as a safer alternative to conventional root canal irrigants. The antibacterial activity of MA-T was assessed against mono-species biofilms (Enterococcus faecalis, Parvimonas micra, Fusobacterium nucleatum), polymicrobial biofilms derived from human supragingival plaque, and an in vitro infected root canal model using E. faecalis. Antibacterial efficacy was evaluated using viable cell counting and confocal laser scanning microscopy (CLSM) to quantify the reduction in bacterial load. Biofilm mass was assessed by crystal violet (CV) staining. Cytotoxicity was evaluated by treating rat osteoblasts and human periodontal ligament fibroblasts with MA-T or NaOCl, followed by assessment of ATP levels and morphological integrity. MA-T eradicated planktonic bacteria, inhibited biofilm formation, and significantly reduced viable bacteria in established biofilms without affecting the total amount of biofilm biomass time-dependently. The infected root canal model showed a substantial decrease in bacterial load. Compared with NaOCl, MA-T-treated cells retained higher ATP levels and normal morphology. MA-T could be a promising root canal irrigant offering effective disinfection and superior biocompatibility, making it a potential alternative to traditional root canal irrigants as NaOCl.
口腔生物膜会导致根尖周炎，这是牙齿缺失的主要原因。本研究评估了一种按需使用的二氧化氯水溶液“匹配转化系统”（MA-T）作为传统根管冲洗剂更安全替代品的潜力。评估了MA-T对单一菌种生物膜（粪肠球菌、微小单胞菌、具核梭杆菌）、源自人类龈上菌斑的多菌种生物膜以及使用粪肠球菌的体外感染根管模型的抗菌活性。使用活菌计数和共聚焦激光扫描显微镜（CLSM）评估抗菌效果，以量化细菌载量的减少。通过结晶紫（CV）染色评估生物膜量。通过用MA-T或次氯酸钠处理大鼠成骨细胞和人牙周膜成纤维细胞，然后评估ATP水平和形态完整性来评估细胞毒性。MA-T可根除浮游细菌，抑制生物膜形成，并随时间依赖性地显著减少已形成生物膜中的活菌数量，而不影响生物膜总生物量。感染根管模型显示细菌载量大幅降低。与次氯酸钠相比，经MA-T处理的细胞保留了更高的ATP水平和正常形态。MA-T可能是一种有前景的根管冲洗剂，能提供有效的消毒和卓越的生物相容性，使其成为次氯酸钠等传统根管冲洗剂的潜在替代品。
