Blair E B, Bretherton W W, Tull A H
Appl Microbiol. 1972 Apr;23(4):826. doi: 10.1128/am.23.4.826-826.1972.
Sterilization and optimal staining of Mycobacterium tuberculosis in sputum smears was accomplished by heating at 65 C for 30 min followed by exposure for 10 min to 10% formaldehyde fumes.
痰涂片结核分枝杆菌的灭菌和最佳染色是通过在65℃加热30分钟,然后暴露于10%甲醛烟雾中10分钟来完成的。