Allen B W
J Clin Pathol. 1981 Jul;34(7):719-22. doi: 10.1136/jcp.34.7.719.
Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between preparation and staining is unavoidable, it is recommended that smears are given additional treatment to prevent the survival of tubercle bacilli.
用一种能检查整个涂片的玻片培养技术检测出了经热固定后存活的结核杆菌。传统的火焰固定法和使用可控热板都未能使结核痰液涂片安全地进行后续处理。用酚-金胺法染色的涂片在培养时未生长出菌落。如果制片和染色之间不可避免地会有延迟，建议对涂片进行额外处理以防止结核杆菌存活。
