用于灭活和固定结核分枝杆菌未染色涂片制剂以提高实验室安全性的方法。
Method for inactivating and fixing unstained smear preparations of mycobacterium tuberculosis for improved laboratory safety.
作者信息
Chedore Pamela, Th'ng Cecelia, Nolan Dennis H, Churchwell George M, Sieffert David E, Hale Yvonne M, Jamieson Frances
机构信息
Laboratories Branch, Ontario Ministry of Health and Long-Term Care, Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 2002 Nov;40(11):4077-80. doi: 10.1128/JCM.40.11.4077-4080.2002.
Abstract
The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.
摘要
在从生物安全柜中取出材料之前，对含有结核分枝杆菌的涂片进行显微镜检查灭活是防止结核病潜在传播给实验室工作人员的一个重要安全因素。研究了热火焰、70%乙醇以及乙醇中1%、3%和5%苯酚对含有结核分枝杆菌涂片的固定和灭活特性。热火焰未能使涂片材料灭活，而乙醇中5%的苯酚成功固定并灭活了来自浓缩痰液样本和培养材料的所有含有结核分枝杆菌的涂片。
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