Leng L, Bajo M, Várady J, Szányiová M
Vet Med (Praha). 1977 Jun;22(6):349-55.
We constructed a modified perfusion apparatus and elaborated a method of extracorporal perfusion of the rumen of sheep. As perfusates we used the bovine plasma diluted in a ratio of 1:1 of an isotonic sodium chloride (NaCl) solution and the whole autologous blood. Transaminases GOT and GPT, ammonia and pH were determined in the perfusate. The different perfusions were evaluated according to previously determined perfusion conditions and criteria. A subject for discussion is the question of suitability of the parameters under examination for judging the state of the perfused organ. The described method is suitable for the study of metabolical processes in the rumen wal.
我们构建了一种改良的灌注装置，并阐述了绵羊瘤胃体外灌注的方法。作为灌注液，我们使用了按等渗氯化钠（NaCl）溶液与全自体血液1:1比例稀释的牛血浆。测定了灌注液中的转氨酶GOT和GPT、氨及pH值。根据先前确定的灌注条件和标准对不同的灌注进行评估。一个值得讨论的问题是所检测参数对于判断被灌注器官状态的适用性问题。所描述的方法适用于瘤胃壁代谢过程的研究。
