Vasiurenko Z P, Siniak K M, Korotich A S, Antonova L A
Zh Mikrobiol Epidemiol Immunobiol. 1977 Aug(8):53-9.
Gas chromatographic method was applied to the study of the fatty acid composition (in Br. melitensis, Br. abortus, Br. suis, and Br. ovis strains. Fatty acid composition was similar in the mentioned brucellae species, except Br. suis No. 1330 significantly differing by this sign. Methyleneoctadecanoic acid content was considerably elevated, and that of octadecenoic -- reduced in brucellae grown on liver agar with the addition of serum and on meat-peptone agar in comparison with brucellae grown on liver agar; apparently this represents one of the mechanisms of the microorganism adaptation to the less favourable conditions of the nutrient medium. Passage of Br. ovis strain through the guinea pig organism led to the appearance of brucellae forming two types of colonies when grown on liver agar with the addition of serum. The fatty acid composition of brucellae forming small transparent colonies was the same as that of the initial culture with the prevalence of methyleneoctadecanoic acid; as to brucellae with larger colonies with irregular margin and nontransparent centre of the colony--octadecenoic acid prevailed in their fatty acid composition, i.e. their composition was similar to such in brucellae of the melitensis and abortus species grown on liver agar.
采用气相色谱法对马尔他布鲁氏菌、流产布鲁氏菌、猪布鲁氏菌和绵羊布鲁氏菌菌株的脂肪酸组成进行了研究。上述布鲁氏菌属的脂肪酸组成相似，但1330号猪布鲁氏菌在这方面有显著差异。与在肝琼脂上生长的布鲁氏菌相比，添加血清的肝琼脂和肉蛋白胨琼脂上生长的布鲁氏菌中亚甲基十八烷酸含量显著升高，而十八碳烯酸含量降低；显然，这是微生物适应营养培养基不利条件的机制之一。绵羊布鲁氏菌菌株通过豚鼠机体传代后，在添加血清的肝琼脂上生长时会出现形成两种菌落类型的布鲁氏菌。形成小透明菌落的布鲁氏菌的脂肪酸组成与初始培养物相同，以亚甲基十八烷酸为主；而形成边缘不规则、菌落中心不透明的较大菌落的布鲁氏菌，其脂肪酸组成以十八碳烯酸为主，即其组成与在肝琼脂上生长的马尔他布鲁氏菌和流产布鲁氏菌的组成相似。
