通过细胞脂肪酸的气液色谱分析鉴别绵羊布鲁氏菌和流产布鲁氏菌。
Differentiation of Brucella ovis from Brucella abortus by gas-liquid chromatographic analysis of cellular fatty acids.
作者信息
Coloe P J, Sinclair A J, Slattery J F, Burke D
出版信息
J Clin Microbiol. 1984 Jun;19(6):896-8. doi: 10.1128/jcm.19.6.896-898.1984.
Abstract
The cellular fatty acid composition of Brucella ovis and Brucella abortus strains was determined by gas-liquid chromatography. Both species were characterized by the presence of fatty acids 16:0, 17:0, 17:0 cyclopropane, 18:0, 18:1, and 19:0 cyclopropane; B. ovis also contained some 15:0. There were differences in the relative proportions of the fatty acids present, and it was possible to differentiate B. ovis from B. abortus on the basis of the absence of 15:0, lower concentrations of 17:0 and 18:1, and higher concentrations of 19:0 cyclopropane in B. abortus. The data indicate that analysis of cellular fatty acid composition by gas-liquid chromatography can be used for the identification of B. ovis and its differentiation from B. abortus.
摘要
采用气液色谱法测定了绵羊布鲁氏菌和流产布鲁氏菌菌株的细胞脂肪酸组成。这两个菌种的特征均为含有脂肪酸16:0、17:0、17:0环丙烷、18:0、18:1和19:0环丙烷；绵羊布鲁氏菌还含有一些15:0。所存在的脂肪酸的相对比例存在差异，并且可以基于流产布鲁氏菌中不存在15:0、17:0和18:1的浓度较低以及19:0环丙烷的浓度较高来区分绵羊布鲁氏菌和流产布鲁氏菌。数据表明，通过气液色谱法分析细胞脂肪酸组成可用于绵羊布鲁氏菌的鉴定及其与流产布鲁氏菌的区分。
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Differentiation of Brucella ovis from Brucella abortus by gas-liquid chromatographic analysis of cellular fatty acids.通过细胞脂肪酸的气液色谱分析鉴别绵羊布鲁氏菌和流产布鲁氏菌。
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