Raad I, Rand K, Gaskins D
Department of Medicine (Infectious Diseases), University of Florida, Gainesville.
J Clin Microbiol. 1990 Jul;28(7):1671-2. doi: 10.1128/jcm.28.7.1671-1672.1990.
A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar. Sixteen strains of various Brucella species were inoculated on BCYE agar; selective BCYE agar with polymyxin, anisomycin, and cefamandole; and brucella blood agar. The growth and recovery rates on the three media tested were comparable for 14 strains. BCYE agar with polymyxin, anisomycin, and cefamandole may be useful as a selective medium for the isolation of brucellae.
一名由流产布鲁氏菌引起慢性骨髓炎的患者凝集效价呈阴性。由于叠加了葡萄球菌感染，导致该菌在非选择性培养基上过度生长，布鲁氏菌仅在选择性缓冲活性炭酵母浸出液（BCYE）琼脂上分离出来。将16株不同布鲁氏菌属菌株接种于BCYE琼脂、含多粘菌素、茴香霉素和头孢孟多的选择性BCYE琼脂以及布鲁氏菌血琼脂上。在所测试的三种培养基上，14株菌株的生长和回收率相当。含多粘菌素、茴香霉素和头孢孟多的BCYE琼脂可用作分离布鲁氏菌的选择性培养基。
