多巴胺、去甲肾上腺素和毛果芸香碱对培养的大鼠垂体肿瘤细胞催乳素分泌和合成的抑制作用。

Inhibition of prolactin secretion and synthesis by dopamine, noradrenaline and pilocarpine in cultured rat pituitary tumour cells.

作者信息

Bang S, Gautvik K M

出版信息

Acta Pharmacol Toxicol (Copenh). 1977 Oct;41(4):317-27. doi: 10.1111/j.1600-0773.1977.tb02671.x.

DOI:10.1111/j.1600-0773.1977.tb02671.x

Abstract

Clonal strains of rat pituitary tumour cells (GH3-cells) synthesize and secrete prolactin into a chemically defined culture medium. Short time treatment (0.5-2 hrs) of cell cultures with noradrenaline (10(-3) M) and dopamine (10(-3 M) reduced the spontaneous secretion of prolactin by 50% and 30%, respectively, while pilocarpine (10(-3) M) had no effect. Long-term treatment (20 hrs) with noradrenaline (10(-3)M) or with pilocarpine (10(-3) M) inhibited prolactin synthesis by 45% and 65% of control cultures, respectively. Neither compounds affected cell growth. The inhibitory effect of noradrenaline, but not that of pilocarpine, was completely reversed 4 hrs after cessation of treatment. Adrenaline, dopamine and acetylcholine in concentrations up to 10(-3) M did not change prolactin synthesis. In contrast thyroliberin treatment (2 X 10(-8)M) caused a 45% increase in prolactin secretion, and resulted in a 40% increase in hormone synthesis after treatment for 20 hrs. It is concluded that both noradrenaline and dopamine are able to inhibit prolactin section. Prolactin synthesis could be inhibited by noradrenaline and pilocarpine. How, only the effect of noradrenaline is easily reversible on cessation of treatment.

摘要

大鼠垂体瘤细胞（GH3细胞）的克隆株可在化学成分明确的培养基中合成并分泌催乳素。用去甲肾上腺素（10⁻³M）和多巴胺（10⁻³M）对细胞培养物进行短时间处理（0.5 - 2小时），可分别使催乳素的自发分泌减少50%和30%，而毛果芸香碱（10⁻³M）则无作用。用去甲肾上腺素（10⁻³M）或毛果芸香碱（10⁻³M）进行长期处理（20小时），分别抑制催乳素合成量为对照培养物的45%和65%。两种化合物均不影响细胞生长。去甲肾上腺素的抑制作用在处理停止4小时后可完全逆转，而毛果芸香碱则不能。浓度高达10⁻³M的肾上腺素、多巴胺和乙酰胆碱均不改变催乳素的合成。相反，促甲状腺素释放激素处理（2×10⁻⁸M）可使催乳素分泌增加45%，处理20小时后激素合成增加40%。结论是，去甲肾上腺素和多巴胺均能抑制催乳素分泌。去甲肾上腺素和毛果芸香碱均可抑制催乳素合成。然而，只有去甲肾上腺素的作用在处理停止后易于逆转。