Poutrel B
Ann Microbiol (Paris). 1977 Apr;128A(3):297-302.
The validity of quantitative determination of protein A by radial immunodiffusion has been tested using human serum, human IgG and porcine serum. The best results are obtained with porcine serum. Maximal sensitivity is obtained after 48 h of incubation with 15 microliters of porcine serum per 13 ml of gel. For these particular conditions the range of linearity varies between 20 and 300 microgram/ml.
已使用人血清、人免疫球蛋白G（IgG）和猪血清对通过放射免疫扩散法定量测定蛋白A的有效性进行了测试。使用猪血清可获得最佳结果。每13毫升凝胶中加入15微升猪血清孵育48小时后可获得最大灵敏度。对于这些特定条件，线性范围在20至300微克/毫升之间。
