Johne B, Jarp J
National Veterinary Institute, Oslo, Norway.
APMIS. 1988 Jan;96(1):43-9.
Monosized magnetic polystyrene beads, Dynabeads M-450, coated with sheep IgG were used in an agglutination test for the detection of cell-surface protein-A, based on Fc-protein-A binding. Ninety-three per cent of the bovine Staphylococcus aureus strains isolated from clinical mastitis expressed surface protein-A. The agglutination test was rapid compared to FITC-labelled IgG binding or dog-serum agar precipitation test for protein-A, and was significantly correlated with these methods. Scanning electron micrographs demonstrated binding of protein-A positive bacteria to the particle surface, thus bridging the beads and forming the agglutination lattice. The microsphere agglutination assay described is a quick, simple and reproducible method for detecting surface protein-A in staphylococci.
包被羊免疫球蛋白G的单分散磁性聚苯乙烯微珠（Dynabeads M-450）用于基于Fc-蛋白A结合的细胞表面蛋白A检测的凝集试验。从临床乳腺炎中分离出的93%的牛金黄色葡萄球菌菌株表达表面蛋白A。与用于检测蛋白A的异硫氰酸荧光素标记的免疫球蛋白G结合试验或犬血清琼脂沉淀试验相比，该凝集试验速度更快，且与这些方法显著相关。扫描电子显微镜照片显示蛋白A阳性细菌与颗粒表面结合，从而使微珠相连并形成凝集晶格。所述微球凝集试验是一种快速、简单且可重复的检测葡萄球菌表面蛋白A的方法。
