Wang Yang, Zhang Lihong, Zhang Zhao, Chen Zhihua, Xu Jingru, Zhang Han, Zheng Haixue, Pei Jingjing
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China.
Virulence. 2025 Dec;16(1):2580141. doi: 10.1080/21505594.2025.2580141. Epub 2025 Oct 31.
T and B lymphocytes protect against the foot and mouth disease virus (FMDV). They are essential for long-term immunity and viral clearance, but their immunological features in FMDV infection remain unknown. Here, we performed single-cell RNA sequencing (scRNA-seq) and conducted integrative analyses to determine cell composition, gene expression, cell-cell communication state, and regulatory network of T and B cells in the spleen of FMDV- and mock-infected mice. Our results revealed variational compositions of immune cells on the subsets level. Additionally, we determined the reshaping of several essential pathways in T and B cells, such as the inhibition of antigen-presentation capacity. Finally, the virus-induced shaping of immune patterns, including cell-cell communication and regulatory networks, was also characterized during FMDV infection. Overall, our research provides valuable insights and resources for understanding the adaptive immune response in FMD.
T淋巴细胞和B淋巴细胞可抵御口蹄疫病毒（FMDV）。它们对于长期免疫和病毒清除至关重要，但其在FMDV感染中的免疫特征仍不清楚。在此，我们进行了单细胞RNA测序（scRNA-seq）并开展综合分析，以确定FMDV感染小鼠和模拟感染小鼠脾脏中T细胞和B细胞的细胞组成、基因表达、细胞间通讯状态及调控网络。我们的结果揭示了免疫细胞在亚群水平上的变化组成。此外，我们还确定了T细胞和B细胞中几条关键信号通路的重塑情况，比如抗原呈递能力受到抑制。最后，我们还对FMDV感染期间病毒诱导的免疫模式塑造进行了表征，包括细胞间通讯和调控网络。总体而言，我们的研究为理解口蹄疫中的适应性免疫反应提供了有价值的见解和资源。
