Bartels P C, Goedemans W T, Roijers A F
Clin Chim Acta. 1977 Nov 15;81(1):63-73. doi: 10.1016/0009-8981(77)90414-4.
A thyroxine radioimmunoassay procedure (T4RIA) based on incubation and separation on Sephadex columns is presented. The assay is rapid and easily to perform; if necessary columns may be regenerated. The raising of antibodies against thyroxine in goats is described in detail. The specificity of the antiserum towards the coupling of several compounds related to thyroxine has been tested. Influence of some buffers on the binding of thyroxine to serum proteins has been investigated. The results of T4RIA in patient sera were in agreement with those obtained by a competitive binding method. The within-day variation was approximately 4% (coefficient of variation, C.V.); day-to-day variation was 7% C.V.
介绍了一种基于在葡聚糖凝胶柱上进行孵育和分离的甲状腺素放射免疫分析方法（T4RIA）。该分析方法快速且易于操作；如有必要，柱子可以再生。详细描述了在山羊体内产生抗甲状腺素抗体的过程。测试了抗血清对几种与甲状腺素相关化合物偶联物的特异性。研究了一些缓冲液对甲状腺素与血清蛋白结合的影响。患者血清中T4RIA的结果与通过竞争结合法获得的结果一致。日内变异约为4%（变异系数，C.V.）；日间变异为7% C.V.
