Goedemans W T, Bartels P C, Roijers A F
Clin Chem. 1977 Dec;23(12):2324-8.
We describe a reproducible radioimmunoassay, with use of Sephadex columns, for measuring normalized thyroxine. In comparison with a competitive protein-binding procedure, the present method is more specific because antibody rather than thyroxine-binding globulin is responsible for the competition between endogeneous and tracer thyroxine. In barbital buffer, various concentrations of thyroxine binding pre-albumin and albumin had no influence on the results. Values obtained by the present method correlated well with those by the free thyroxine index and a generally accepted thyroxine normalized method.
我们描述了一种使用葡聚糖凝胶柱测量标准化甲状腺素的可重复放射免疫测定法。与竞争性蛋白结合法相比，本方法更具特异性，因为是抗体而非甲状腺素结合球蛋白参与内源性甲状腺素与示踪甲状腺素之间的竞争。在巴比妥缓冲液中，不同浓度的甲状腺素结合前白蛋白和白蛋白对结果无影响。本方法获得的值与游离甲状腺素指数及一种普遍接受的甲状腺素标准化方法所得的值相关性良好。
