用竞争性蛋白结合法测定血清甲状腺素：一次性葡聚糖凝胶柱的重复使用

Assay of serum thyroxine by competitive protein binding: reuse of disposable sephadex columns.

作者信息

Bastomsky C H, Wyse J M, Murthy P V

出版信息

Clin Biochem. 1976 Apr;9(2):89-92. doi: 10.1016/s0009-9120(76)80024-0.

Abstract

A method for the assay of total serum thyroxine on reusable Sephadex G-25 fine columns, obtained from the Ames Tetralute kit, and competitive protein binding with TBG is described. The columns are regenerated with diluted plasma and may be used for at least 3 months. The procedure is rapid and simple and requires no extraction, centrifugation or evaporation. Recovery of added thyroxine from serum is essentially complete. The between-assay and within-assay coefficients of variation are about 5.5%. Correlation with values obtained using the Tetralute kit was good (r = 0.988). Values in 75 euthyroid subjects were normally distributed and therefore the normal range was taken as the mean +/- 2 SD or 4.6-10.4 mug/100 ml.

摘要

本文描述了一种使用从艾姆斯四碘甲状腺原氨酸试剂盒获得的可重复使用的葡聚糖凝胶G-25细柱测定血清总甲状腺素的方法，以及与甲状腺素结合球蛋白的竞争性蛋白结合。这些柱子用稀释的血浆再生，可使用至少3个月。该方法快速简单，无需萃取、离心或蒸发。血清中添加的甲状腺素回收率基本完全。批间和批内变异系数约为5.5%。与使用四碘甲状腺原氨酸试剂盒获得的值相关性良好（r = 0.988）。75名甲状腺功能正常受试者的值呈正态分布，因此正常范围取为平均值±2标准差，即4.6-10.4微克/100毫升。