蜡样芽孢杆菌T孢子经三氯乙酸处理后的延迟萌发及其通过加热的再激活。

Delayed germination of Bacillus cereus T spores after treatment with trichloroacetic acid and their reactivation by heating.

作者信息

Shibata H, Murakami H, Tani I

出版信息

Microbiol Immunol. 1980;24(4):291-8. doi: 10.1111/j.1348-0421.1980.tb02832.x.

DOI:10.1111/j.1348-0421.1980.tb02832.x

Abstract

Treatment of Bacillus cereus T spores with trichloroacetic acid delayed their germination. The extent of retardation depended on the concentration of trichloroacetic acid, and the temperature, pH and duration of treatment. The effect was completely reversed by subsequent heating, and this restoration of germination also depended on the temperature and duration of heat treatment. Fourteen compounds were examined for their ability to suppress germination of spores. The halogenated fatty acids tested, such as trifluoro-, tribromo-, and dichloroacetic acid, caused suppression of germination, whereas other compounds, i.e., free fatty acids and amino acids, did not. It is concluded that the charge distribution of fatty acid molecules is important for their effect in suppressing germination of spores.

摘要

用三氯乙酸处理蜡样芽孢杆菌T孢子会延迟其萌发。延迟程度取决于三氯乙酸的浓度、处理温度、pH值和持续时间。随后加热可完全逆转这种效应，而这种萌发恢复也取决于热处理的温度和持续时间。检测了14种化合物抑制孢子萌发的能力。所测试的卤代脂肪酸，如三氟乙酸、三溴乙酸和二氯乙酸，会抑制萌发，而其他化合物，即游离脂肪酸和氨基酸则不会。得出的结论是，脂肪酸分子的电荷分布对其抑制孢子萌发的作用很重要。