Nishioka K, Romsdahl M M, Fritsche H A, McMurtrey M J
Int J Cancer. 1977 Nov 15;20(5):689-93. doi: 10.1002/ijc.2910200507.
Serum tyrosinase activity has been measured by adapting the [3]tyrosine assay for tyrosinase and significant elevations of serum tyrosinase activity were found in patients with malignant melanoma. In contrast to findings in a study which utilized [14C]tyrosine, augmented levels of tyrosinase activity were not observed in sera from patients with other malignancies, including subjects with carcinoma of the breast. The results of the examinations for soluble tyrosinase activity in human malignant melanoma tissue-cultured lines were all positive, whereas human cell lines from carcinoma of the breast, carcinoma of the colon and sarcoma uniformly showed no activity. The method employed for detecting tyrosinase activity holds promise as a specific diagnostic test and may be valuable for monitoring the response to clinical treatment of patients with malignant melanoma.
通过采用针对酪氨酸酶的[3]酪氨酸测定法来测量血清酪氨酸酶活性，发现恶性黑色素瘤患者的血清酪氨酸酶活性显著升高。与一项使用[14C]酪氨酸的研究结果相反，在包括乳腺癌患者在内的其他恶性肿瘤患者的血清中未观察到酪氨酸酶活性水平升高。对人恶性黑色素瘤组织培养细胞系中可溶性酪氨酸酶活性的检测结果均为阳性，而来自乳腺癌、结肠癌和肉瘤的人细胞系均未显示出活性。用于检测酪氨酸酶活性的方法有望成为一种特异性诊断测试，并且对于监测恶性黑色素瘤患者的临床治疗反应可能具有重要价值。
