Libich M
Immunology. 1972 Nov;23(5):785-90.
By means of migration inhibition tests a new antigenic specificity was demonstrated in rabbit and hamster embryonal fibroblasts cultured in the presence of -fluorophenylalanine; a similar result was obtained in the cultivation of the cells in the presence of another amino acid analogue (S-benzylcysteine). Changes of antigenic properties of the cells induced by a definitive analogue were immunologically specific for the particular analogue. In rabbits immunized with rabbit embryonal fibroblasts cultured in the presence of -fluorophenylalanine only delayed type hypersensitivity against the employed cells was demonstrated; antibody production could not so far be demonstrated. It is probable that the altered antigenic specificity of cellular proteins was induced by analogue incorporation into the newly synthesized protein components, which resulted in an altered conformation of protein molecules.
通过迁移抑制试验，在含氟苯丙氨酸培养的兔和仓鼠胚胎成纤维细胞中证实了一种新的抗原特异性；在另一种氨基酸类似物（S-苄基半胱氨酸）存在下培养细胞时也得到了类似结果。特定类似物诱导的细胞抗原特性变化对该特定类似物具有免疫特异性。在用含氟苯丙氨酸培养的兔胚胎成纤维细胞免疫的兔中，仅表现出对所用细胞的迟发型超敏反应；目前尚未证实有抗体产生。细胞蛋白质抗原特异性的改变可能是由于类似物掺入新合成的蛋白质成分中，导致蛋白质分子构象改变所致。
