用于检测牛流产材料中流产布鲁氏菌的直接荧光抗体试验。
The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material.
作者信息
Corbel M J
出版信息
J Hyg (Lond). 1973 Mar;71(1):123-9. doi: 10.1017/s0022172400046283.
Abstract
The direct fluorescent antibody test was assessed for detection of Brucella abortus in bovine abortion material. Br. abortus organisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. Counterstaining with Evans Blue was necessary to suppress tissue auto-fluorescence. The method was specific and enabled brucella infection to be differentiated, inter alia, from Q-fever infection.
摘要
对直接荧光抗体试验进行了评估，以检测牛流产材料中的牛布鲁氏菌。在用荧光抗体染色的涂片上，牛布鲁氏菌很容易被检测为特征性的细胞内团块。用伊文思蓝复染对于抑制组织自发荧光是必要的。该方法具有特异性，尤其能够区分布鲁氏菌感染和Q热感染。
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