Singh S V, Gupta V K, Singh N
Goat Health Division, Central Institute for Research on Goats, Makhdoom, Mathura, UP, India.
Trop Anim Health Prod. 2000 Jun;32(3):155-63. doi: 10.1023/a:1005283531306.
A dot-ELISA (d-ELISA) test was evaluated and compared with the serum agglutination test (SAT), micro-complement fixation test (CFT) and a plate-ELISA (p-ELISA) for field use in screening herds of goats against brucellosis. During the standardization of the dot-ELISA kit on 1732 caprine serum samples, 1571 samples out of 1666 were found to be negative in d-ELISA, SAT and micro-CFT, while 59 were positive in different combinations. Of a further 66 serum samples, 34 were negative and 31 were positive in different combinations in d-ELISA, SAT, micro-CFT and p-ELISA. A total of 1584 goats belonging to different herds were then screened for brucellosis. Of the 694 serum samples screened in the first batch using d-ELISA, a positive reaction was observed in 26 cases. Further screening of these cases revealed 13 and 21 goats as positive reactors in SAT and CFT, respectively. In a second batch of 890 goats there were 109 positive reactors in d-ELISA. Among these 109 goats, 34, 40 and 80 goats were positive reactors in SAT, CFT and p-ELISA, respectively. The results of d-ELISA correlated well with those of p-ELISA. Dot-ELISA was found to be a more suitable and rapid test for screening large numbers of goats in the field.
对一种斑点酶联免疫吸附测定（d-ELISA）进行了评估，并与血清凝集试验（SAT）、微量补体结合试验（CFT）和平板酶联免疫吸附测定（p-ELISA）进行比较，以用于现场筛查山羊群中的布鲁氏菌病。在使用1732份山羊血清样本对d-ELISA试剂盒进行标准化的过程中，1666份样本中有1571份在d-ELISA、SAT和微量CFT中呈阴性，而59份在不同组合中呈阳性。在另外66份血清样本中，34份在d-ELISA、SAT、微量CFT和p-ELISA中呈阴性，31份在不同组合中呈阳性。然后对来自不同羊群的总共1584只山羊进行了布鲁氏菌病筛查。在第一批使用d-ELISA筛查的694份血清样本中，有26例观察到阳性反应。对这些病例的进一步筛查显示，SAT和CFT中分别有13只和21只山羊为阳性反应动物。在第二批890只山羊中，d-ELISA中有109只阳性反应动物。在这109只山羊中，SAT、CFT和p-ELISA中分别有34只、40只和80只山羊为阳性反应动物。d-ELISA的结果与p-ELISA的结果相关性良好。发现斑点酶联免疫吸附测定对于现场筛查大量山羊是一种更合适且快速的检测方法。
