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人乳的DNA聚合酶活性

DNA polymerase activities of human milk.

作者信息

Gerwin B I, Ebert P S, Chopra H C, Smith S G, Kvedar J P, Albert S, Brennan M J

出版信息

Science. 1973 Apr 15;180(4082):198-201. doi: 10.1126/science.180.4082.198.

Abstract

DNA polymerases have been partially purified from human milk. A DNA polymerase detected by phosphocellulose chromatography is similar to the enzymes of RNA tumor viruses in that a hybrid of polyriboadenylate and oligodeoxythymidylate is a better template than is DNA. However, this polymerase differed from that of the RNA tumor viruses in its chromatographic behavior. Three different methods of detecting "reverse transcriptase" activity failed to correlate with the donor's family history of cancer.

摘要

已从人乳中部分纯化出DNA聚合酶。通过磷酸纤维素色谱法检测到的一种DNA聚合酶与RNA肿瘤病毒的酶相似,因为聚核糖腺苷酸和寡聚脱氧胸苷酸的杂交体比DNA是更好的模板。然而,这种聚合酶在色谱行为上与RNA肿瘤病毒的聚合酶不同。三种检测“逆转录酶”活性的不同方法均与捐赠者的癌症家族史无关。

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