Waterhouse C, Abraham G, Vaughan J
J Clin Invest. 1973 May;52(5):1067-77. doi: 10.1172/JCI107272.
The simultaneous administration of labeled human gammaG globulin and L-chains to subjects has allowed examination of the metabolic relationship between a component part and the whole immunoglobulin molecule. Studies were carried out in a series of control subjects and in a group of patients where substandard production of gamma-globulin was anticipated, i.e., patients with the nephrotic syndrome on chronic Imuran therapy and patients with uremia. Full expression of the plasma decay curve was obtained for both substances, that for L-chain requiring only 4-5 days except in uremic subjects and that for gamma-globulin requiring up to 30-40 days. Urinary excretion of inorganic iodide was also quantitated for 20-30 days. Equilibrium of the extravascular, vascular, and urinary radioactivity from the labeled gammaG globulin was usually not seen during this time interval suggesting more than one site of catabolism of the protein. Excess L-chain poduction was about 45% of the total L-chain production and probably in no instance could account for the low gamma-globulin production seen in certain patients with renal disease.
给受试者同时注射标记的人γG球蛋白和轻链，使得对免疫球蛋白分子的组成部分与整个分子之间的代谢关系进行研究成为可能。在一系列对照受试者以及一组预计γ球蛋白产生不足的患者中开展了研究，即接受慢性硫唑嘌呤治疗的肾病综合征患者和尿毒症患者。两种物质均获得了完整的血浆衰变曲线，轻链的曲线除了在尿毒症受试者中只需4 - 5天，而γ球蛋白的曲线则需要长达30 - 40天。还对20 - 30天内的无机碘尿排泄量进行了定量。在此时间间隔内，通常未见标记的γG球蛋白在血管外、血管内和尿液中的放射性达到平衡，这表明该蛋白质的分解代谢不止一个部位。过量轻链产生约占总轻链产生量的45%，在任何情况下都可能无法解释某些肾病患者中出现的γ球蛋白产生不足的情况。
