Bandilla K K, McDuffie F C, Gleich G J
Clin Exp Immunol. 1969 Dec;5(6):627-41.
A method, radio-immunoprecipitation, is described which measures the antibody in the different classes in whole sera by its antigen binding capacity. In nineteen of twenty-four individuals immunized with haemocyanin, haemagglutinating antibodies were produced in the primary response, but in only five were amounts significant enough to measure by radio-immunoprecipitation. In these five individuals, the primary response was characterized by an early appearance of IgM and IgA antibodies; IgG antibody was detectable but only in small amounts and not before the 18th day after immunization. All twenty-four individuals responded to a secondary injection. A sharp rise in IgG antibody after the secondary injection is typical of the secondary response. A definite but much lower anamnestic response for IgM and IgA is observed. The difference is so uniform and striking that it may well be characteristic for some primary responses in man.
本文描述了一种放射免疫沉淀法，该方法通过抗原结合能力来测定全血清中不同类别的抗体。在24名用血蓝蛋白免疫的个体中，有19人在初次反应中产生了血凝抗体，但只有5人的抗体量足以通过放射免疫沉淀法进行测定。在这5名个体中，初次反应的特征是IgM和IgA抗体早期出现；IgG抗体可检测到，但量很少，且在免疫后第18天才出现。所有24名个体对二次注射均有反应。二次注射后IgG抗体急剧上升是二次反应的典型特征。观察到IgM和IgA有明确但低得多的回忆反应。这种差异非常一致且显著，很可能是人类某些初次反应的特征。
