羊瘙痒病病原体的沉降特性。
Sedimentation properties of the scrapie agent.
作者信息
Prusiner S B, Hadlow W J, Eklund C M, Race R E
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4656-60. doi: 10.1073/pnas.74.10.4656.
Abstract
The sedimentation behavior of the scrapie agent in homogenates of spleen from infected mice has been determined. Approximately 90% of the scrapie agent was sedimented at an omega2t value of 3 X 10(10) rad2/sec in a fixed-angle rotor. Sedimentation of the agent was not substantially affected by sonication or by treatment with the detergent sodium deoxycholate. The sedimentation profiles of the scrapie agent were similar to those observed for free polyribosomes, but differed from those exhibited by five other subcellular markers. Comparative studies showed that the sedimentation profiles of subcellular markers in spleen suspensions from mice infected with scrapie did not differ from uninoculated controls. These studies suggest that the scrapie agent is a discrete infectious particle which should be separable from cellular membranes.
摘要
已测定了感染小鼠脾脏匀浆中瘙痒病病原体的沉降行为。在固定角度转头中，约90%的瘙痒病病原体在ω2t值为3×10(10)弧度2/秒时沉降。病原体的沉降基本不受超声处理或脱氧胆酸钠洗涤剂处理的影响。瘙痒病病原体的沉降图谱与游离多核糖体的沉降图谱相似，但与其他五种亚细胞标志物的沉降图谱不同。比较研究表明，感染瘙痒病的小鼠脾脏悬液中亚细胞标志物的沉降图谱与未接种对照无差异。这些研究表明，瘙痒病病原体是一种离散的感染性颗粒，应可与细胞膜分离。
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