无膜瘙痒病活性
Membrane-free scrapie activity.
作者信息
Malone T G, Marsh R F, Hanson R P, Semancik J S
出版信息
J Virol. 1978 Mar;25(3):933-5. doi: 10.1128/JVI.25.3.933-935.1978.
Abstract
Determinations of scrapie activity in subcellular fractions from infected hamster brains through the asymptomatic and symptomatic course of infection revealed the presence of substantial amounts of scrapie infectivity in the 100,000 X g supernatant fractions, indicating that association with physically discernible membrane structures is not necessary for the transmission of the scrapie agent. An increase of scrapie infectivity in the 100,000 X g supernatant fractions after vigorous homogenization of infected membrane-rich fractions suggests that the agent is identical in membrane-rich and 100,000 X g supernatant fractions.
摘要
通过感染仓鼠大脑无症状和有症状感染过程的亚细胞组分中瘙痒病活性的测定，发现在100,000×g上清液组分中存在大量瘙痒病感染性，这表明与物理上可识别的膜结构结合对于瘙痒病病原体的传播不是必需的。对富含膜的感染组分进行剧烈匀浆后，100,000×g上清液组分中瘙痒病感染性增加，这表明富含膜的组分和100,000×g上清液组分中的病原体是相同的。
相似文献
1
Membrane-free scrapie activity.无膜瘙痒病活性
J Virol. 1978 Mar;25(3):933-5. doi: 10.1128/JVI.25.3.933-935.1978.
2
Purification of non-infectious ganglioside preparations from scrapie-infected brain tissue.
Arch Virol. 1992;124(1-2):111-21. doi: 10.1007/BF01314629.
3
Characteristics of a short incubation model of scrapie in the golden hamster.金黄仓鼠瘙痒病短潜伏期模型的特征
J Gen Virol. 1977 Feb;34(2):295-304. doi: 10.1099/0022-1317-34-2-295.
4
Evidence that the transmission of one source of scrapie agent to hamsters involves separation of agent strains from a mixture.
J Gen Virol. 1978 Jun;39(3):487-96. doi: 10.1099/0022-1317-39-3-487.
5
Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis.
Proc Natl Acad Sci U S A. 1990 Sep;87(18):7240-4. doi: 10.1073/pnas.87.18.7240.
6
Molecular hybridization studies with scrapie brain nucleic acids. II. Differential expression in scrapie hamster brain.用羊瘙痒病脑核酸进行的分子杂交研究。II. 羊瘙痒病仓鼠脑中的差异表达
Arch Virol. 1986;88(1-2):79-90. doi: 10.1007/BF01310892.
7
Pathogenesis of scrapie (strain 263K) in hamsters infected intracerebrally, intraperitoneally or intraocularly.仓鼠经脑内、腹腔内或眼内感染羊瘙痒病（毒株263K）的发病机制。
J Gen Virol. 1986 Feb;67 ( Pt 2):255-63. doi: 10.1099/0022-1317-67-2-255.
8
Altered scrapie infectivity estimates by titration and incubation period in the presence of detergents.在存在去污剂的情况下，通过滴定和潜伏期改变瘙痒病感染性估计值。
J Gen Virol. 1983 Sep;64 (Pt 9):2045-50. doi: 10.1099/0022-1317-64-9-2045.
9
Organ-specific modification of the dose-response relationship of scrapie infectivity.
J Infect Dis. 1990 Apr;161(4):783-6. doi: 10.1093/infdis/161.4.783.
10
Levels of infectivity in the blood throughout the incubation period of hamsters peripherally injected with scrapie.外周注射瘙痒病病原体的仓鼠在整个潜伏期内血液中的感染性水平。
Arch Virol. 1989;108(1-2):145-9. doi: 10.1007/BF01313752.
引用本文的文献
1
Heterogeneity and Architecture of Pathological Prion Protein Assemblies: Time to Revisit the Molecular Basis of the Prion Replication Process?病理性朊病毒蛋白聚集体的异质性和结构：重新审视朊病毒复制过程的分子基础的时机到了？
Viruses. 2019 May 10;11(5):429. doi: 10.3390/v11050429.
2
Prion propagation: the role of protein dynamics.朊病毒传播：蛋白质动力学的作用
Prion. 2007 Jan-Mar;1(1):36-43. doi: 10.4161/pri.1.1.3992. Epub 2007 Jan 10.
3
Ultrastructural studies on scrapie prion protein crystals obtained from reverse micellar solutions.从反胶束溶液中获得的瘙痒病朊病毒蛋白晶体的超微结构研究。
Biophys J. 1999 Feb;76(2):1048-62. doi: 10.1016/S0006-3495(99)77270-X.
4
Disruption of prion rods generates 10-nm spherical particles having high alpha-helical content and lacking scrapie infectivity.朊病毒杆状结构的破坏会产生具有高α-螺旋含量且缺乏瘙痒病感染力的10纳米球形颗粒。
J Virol. 1996 Mar;70(3):1714-22. doi: 10.1128/JVI.70.3.1714-1722.1996.
5
Attempts to restore scrapie prion infectivity after exposure to protein denaturants.在暴露于蛋白质变性剂后恢复羊瘙痒病朊病毒感染性的尝试。
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2793-7. doi: 10.1073/pnas.90.7.2793.
6
Experimental scrapie in golden Syrian hamsters: temporal comparison of in vitro cell-fusing activity with brain infectivity and histopathological changes.叙利亚金黄地鼠实验性瘙痒病：体外细胞融合活性与脑传染性及组织病理学变化的时间比较
Infect Immun. 1982 Jul;37(1):195-9. doi: 10.1128/iai.37.1.195-199.1982.
7
Pathogenesis of hamster scrapie. Adherent splenocytes are associated with relatively high levels of infectivity.仓鼠瘙痒病的发病机制。贴壁脾细胞与相对较高水平的传染性相关。
Arch Virol. 1990;112(3-4):283-9. doi: 10.1007/BF01323173.
8
Prion liposomes.朊病毒脂质体
Biochem J. 1990 Feb 15;266(1):1-14. doi: 10.1042/bj2660001.
本文引用的文献
1
THE INTRACELLULAR LOCATION OF THE AGENT OF MOUSE SCRAPIE.小鼠瘙痒病病原体的细胞内定位
J Gen Microbiol. 1964 Feb;34:319-25. doi: 10.1099/00221287-34-2-319.
2
Attempts to release the scrapie agent from tissue debris.从组织碎片中释放痒病病原体的尝试。
J Comp Pathol. 1967 Jul;77(3):301-7. doi: 10.1016/0021-9975(67)90039-4.
3
An experimental examination of the scrapie agent in cell membrane mixtures. I. Stability and physicochemical properties of the scrapie agent.
J Comp Pathol. 1971 Jan;81(1):23-32. doi: 10.1016/0021-9975(71)90051-x.
4
Nature of the scrapie agent.
Nature. 1967 Sep 2;215(5105):1041-3. doi: 10.1038/2151041a0.
5
Centrifugation studies on the infectivities of cellular fractions derived from mouse brain infected with scrapie (Suffolk strain).对感染羊瘙痒病（萨福克毒株）的小鼠脑组织细胞组分的感染性进行的离心研究。
J Gen Microbiol. 1965 Jul;40(1):71-9. doi: 10.1099/00221287-40-1-71.
6
An experimental examination of the scrapie agent in cell membrane mixtures. II. The association of scrapie activity with membrane fractions.
J Comp Pathol. 1971 Apr;81(2):255-65. doi: 10.1016/0021-9975(71)90100-9.
7
Comparison of scrapie and transmissible mink encephalopathy in hamsters. I. Biochemical studies of brain during development of disease.仓鼠中羊瘙痒病与传染性水貂脑病的比较。I. 疾病发展过程中大脑的生化研究。
J Infect Dis. 1975 Feb;131(2):97-103. doi: 10.1093/infdis/131.2.97.
8
The membrane location of scrapie infectivity.瘙痒病感染性的膜定位。
J Gen Virol. 1976 Jun;31(3):441-5. doi: 10.1099/0022-1317-31-3-441.
9
Partial purification of the scrapie agent from mouse brain by pressure disruption and zonal centrifugation in sucrose-sodium chloride gradients.通过压力破碎以及在蔗糖 - 氯化钠梯度中进行区带离心，从小鼠脑中对瘙痒病病原体进行部分纯化。
Virology. 1976 Mar;70(1):230-7. doi: 10.1016/0042-6822(76)90261-0.
10
Properties of the scrapie agent-endomembrane complex from hamster brain.来自仓鼠脑的瘙痒病病原体-内膜复合体的特性
J Virol. 1976 May;18(2):693-700. doi: 10.1128/JVI.18.2.693-700.1976.
Zotero 插件