Lepoint A
Virchows Arch B Cell Pathol. 1977 Oct 7;25(1):53-60. doi: 10.1007/BF02889420.
Mouse Ehrlich ascites tumor cells were centrifuged at low speed on a linear Ficoll gradient. Cells from different fractions of the gradient were collected separately and analysed by cytological and cytochemical methods (DNA content, mean volume and number of cells). Nearly pure populations of cells in G1 or S were selected. A heterogeneous population containing 70% G2 and mitotic cells was also isolated. No ultrastructural alterations were detected in the cells after centrifugation. Selected G1 cells were cultured in vitro, their kinetic parameters were measured and compared with those of the original population. No difference was observed as far as the duration of the cycle of these cells is concerned.
将小鼠艾氏腹水瘤细胞在线性菲可梯度上进行低速离心。分别收集梯度不同组分中的细胞，并通过细胞学和细胞化学方法（DNA含量、平均体积和细胞数量）进行分析。挑选出处于G1期或S期的近乎纯的细胞群体。还分离出了一个含有70% G2期和有丝分裂期细胞的异质群体。离心后未在细胞中检测到超微结构改变。挑选出的G1期细胞进行体外培养，测量其动力学参数并与原始群体的参数进行比较。就这些细胞的周期持续时间而言，未观察到差异。
