Rigau-Lloveras J, Grau-Oliete M R, Rivera-Fillat M P
Rev Esp Fisiol. 1984 Mar;40(1):87-94.
Exponentially growing L5178Y cells in suspension culture were separated according to their position in the cell cycle on the basis of their volume with a velocity sedimentation method in which a linear and continuous ficoll gradient was used. Highly purified populations of G1 and S cells were obtained, containing about 90% G1 phase cells and 80% S phase cells. The method is rapid and a larger number of cells can be easily processed with no loss of viability.
采用速度沉降法，利用线性连续的聚蔗糖梯度，根据悬浮培养中指数生长的L5178Y细胞的体积，依据其在细胞周期中的位置对其进行分离。获得了高度纯化的G1期和S期细胞群体，其中G1期细胞约占90%，S期细胞约占80%。该方法速度快，可轻松处理大量细胞且不损失活力。
