Bollin E, Sulkowski E
Prep Biochem. 1979;9(4):339-58. doi: 10.1080/00327487908061699.
The glycoprotein nature of Syrian hamster interferon was tested on several immobilized lectins. The specific retention of a small portion (20%) of interferon activity was observed only on concanavalin A-agarose; Component I of the interferon (not retained) has an apparent molecular weight of 23,500 whereas Component II (retained) is larger, 31,500. The apparent hydrophobicity of Syrian hamster interferon was probed by its chromatography on: (a) straight chain hydrocarbons of varied length; (b) aromatic ligands (aminobenzene, benzylamine, beta-phenylethylamine, gamma-phenyl-propylamine); ligands listed in (a) and (b) were immobilized to cyanogen bromide-activated agarose (isourea linkage); and (c) phenyl-agarose (Phenyl-Sepharose CL-4B), an aromatic ligand immobilized via a 2-hydroxypropyl arm to the agarose (ether linkage).
在几种固定化凝集素上对叙利亚仓鼠干扰素的糖蛋白性质进行了测试。仅在伴刀豆球蛋白A-琼脂糖上观察到一小部分（20%）干扰素活性的特异性保留；干扰素的组分I（未保留）的表观分子量为23,500，而组分II（保留）更大，为31,500。通过以下色谱法探究了叙利亚仓鼠干扰素的表观疏水性：（a）不同长度的直链烃；（b）芳香配体（氨基苯、苄胺、β-苯乙胺、γ-苯丙胺）；（a）和（b）中列出的配体被固定到溴化氰活化的琼脂糖上（异脲键）；以及（c）苯基琼脂糖（苯基-琼脂糖CL-4B），一种通过2-羟丙基臂固定到琼脂糖上的芳香配体（醚键）。
