An T, Sell S
Immunology. 1973 Feb;24(2):277-88.
Using single-coating indirect rosette formation (SIRF), a modification of the mixed antiglobulin reaction, the percentage of rabbit peripheral blood lymphocytes with demonstrable a1 immunoglobulin allotypic determinants is far smaller than the percentage with b4 or b5 allotypic determinants. The number of lymphocytes with a1 determinants, which are located on the Fd portion of the immunoglobulin molecule, is consistently increased (two to eight-fold) using double-coating indirect rosette formation (DIRF). In contrast, the percentage of lymphocytes with b4 or b5 determinants, which are located on the immunoglobulin light chains, remains the same or is only slightly increased, so that the lymphocytes from four a1a1 b5b5 rabbits, when tested separately for a1 and b5 by DIRF, have similar percentages of a1 and b5 containing cells. SIRF lymphocytes are formed by coating lymphocytes with antiallotypic antibody which will bind to erythrocytes coated with subagglutinating doses of anti-RBC antibody which carries the same allotypic specificity as the lymphocyte donor. DIRF lymphocytes are formed by interposing two antiallotypic molecules between the lymphocyte and the indicator red cell. For example, b4 lymphocytes coated with b5 anti-b4 molecules are double coated by the addition of anti-b5 made in a b6 rabbit and rosettes formed by the addition of sheep red cells coated with b5 anti-RBC. The consistent increase in the numbers of lymphocytes with a1 allotypic determinants by DIRF as compared to b group (b4 or b5) determinants suggests that the Fd portion of lymphocyte surface immunoglobulins is less exposed than light chains or the Fc piece.
使用单包被间接玫瑰花结形成法（SIRF），即混合抗球蛋白反应的一种改良方法，可证明具有a1免疫球蛋白同种异型决定簇的兔外周血淋巴细胞百分比远低于具有b4或b5同种异型决定簇的淋巴细胞百分比。使用双包被间接玫瑰花结形成法（DIRF）时，位于免疫球蛋白分子Fd部分的具有a1决定簇的淋巴细胞数量持续增加（增加两到八倍）。相比之下，位于免疫球蛋白轻链上的具有b4或b5决定簇的淋巴细胞百分比保持不变或仅略有增加，因此，当通过DIRF分别检测来自四只a1a1 b5b5兔的淋巴细胞的a1和b5时，含a1和b5的细胞百分比相似。SIRF淋巴细胞是通过用抗同种异型抗体包被淋巴细胞形成的，该抗同种异型抗体将与用亚凝集剂量的抗红细胞抗体包被的红细胞结合，该抗红细胞抗体与淋巴细胞供体具有相同的同种异型特异性。DIRF淋巴细胞是通过在淋巴细胞和指示红细胞之间插入两个抗同种异型分子形成的。例如，用b5抗b4分子包被的b4淋巴细胞通过添加在b6兔中制备的抗b5进行双包被，并通过添加用b5抗红细胞包被的绵羊红细胞形成玫瑰花结。与b组（b4或b5）决定簇相比，DIRF导致具有a1同种异型决定簇的淋巴细胞数量持续增加，这表明淋巴细胞表面免疫球蛋白的Fd部分比轻链或Fc片段暴露程度更低。
