链球菌M蛋白对调理素化前期与免疫沉淀的型特异性抑制作用
Type-specific inhibition of preopsonization versus immunoprecipitation by Streptococcal M proteins.
作者信息
Beachey E H, Cunningham M
出版信息
Infect Immun. 1973 Jul;8(1):19-24. doi: 10.1128/iai.8.1.19-24.1973.
Abstract
Purified M protein of group A streptococci inhibits in vitro phagocytosis of bacteria nonspecifically when it is mixed directly with the test blood. We devised a method of preopsonization inhibition that avoids direct contact between the test blood and the opsonic inhibitory agent. Each of the M proteins tested by this method inhibited opsonization and phagocytosis of only homologous-type streptococci. Titration of the M antigen in various purified preparations demonstrated a clear dissociation between titers of type-specific precipitating M antigen and type-specific opsonic inhibitory M antigen. Since opsonization reflects protective antibody, assays of M protein based on reaction with opsonic antibody should reflect more accurately the presence of the type-specific M determinant involved in protective immunity against streptococcal infections.
摘要
当A组链球菌的纯化M蛋白直接与测试血液混合时，它会非特异性地抑制体外细菌的吞噬作用。我们设计了一种调理素抑制方法，避免测试血液与调理素抑制剂直接接触。用这种方法测试的每种M蛋白仅抑制同源型链球菌的调理作用和吞噬作用。对各种纯化制剂中M抗原的滴定表明，型特异性沉淀M抗原的滴度与型特异性调理素抑制M抗原的滴度之间存在明显的解离。由于调理作用反映了保护性抗体，基于与调理素抗体反应的M蛋白检测应能更准确地反映参与抗链球菌感染保护性免疫的型特异性M决定簇的存在。
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