大鼠实验性变应性脑脊髓炎易感性与主要组织相容性位点的连锁关系。
Linkage of susceptibility to experimental allergic encephalomyelitis to the major histocompatibility locus in the rat.
作者信息
Williams R M, Moore M J
出版信息
J Exp Med. 1973 Oct 1;138(4):775-83. doi: 10.1084/jem.138.4.775.
Experimental allergic encephalomyelitis (EAE) was induced in rats of various genotypes by injection of 10 microg guinea pig basic protein in complete Freund's adjuvant containing 100 microg H(37) RV M. tuberculosis. Histologically verified EAE was present in 20/20 Lewis, 17/17 (Lewis x BN)F(1), 9/9 Lewis backcross, and 21/42 BN backcross rats. Among the BN backcross animals, 25/42 were determined to carry the major histocompatibility type characteristic of the Lewis strain and 21 of these had EAE. Separate groups of Lewis, BN, and (Lewis x BN)F(1) rats were immunized as described and skin tested on day 13 with 10 microg guinea pig basic protein and rat S basic protein. Animals of each genotype had Arthus and delayed skin reactivity to both antigens. These data are compatible with the hypothesis that susceptibility to EAE in rats is controlled by an autosomal dominant gene linked to the major histocompatibility locus. It is proposed that this is an immune response gene, designated Ir-EAE, which controls T cell reactivity directed against a highly encephalitogenic portion of the basic protein molecule.
通过在含有100微克结核分枝杆菌H(37) RV的完全弗氏佐剂中注射10微克豚鼠碱性蛋白，在各种基因型的大鼠中诱发实验性变应性脑脊髓炎（EAE）。经组织学证实，20只Lewis大鼠、17只（Lewis×BN）F1大鼠、9只Lewis回交大鼠和21只BN回交大鼠出现了EAE。在BN回交动物中，42只中有25只被确定携带Lewis品系的主要组织相容性类型特征，其中21只患有EAE。将单独的Lewis、BN和（Lewis×BN）F1大鼠组按上述方法进行免疫，并在第13天用10微克豚鼠碱性蛋白和大鼠S碱性蛋白进行皮肤试验。每种基因型的动物对两种抗原均有阿瑟斯反应和迟发性皮肤反应。这些数据与大鼠对EAE的易感性由与主要组织相容性位点连锁的常染色体显性基因控制这一假说相符。有人提出，这是一个免疫反应基因，命名为Ir-EAE，它控制针对碱性蛋白分子高度致脑炎性部分的T细胞反应性。
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