诺卡氏菌抗酸性的吡啶提取法
Pyridine extraction of nocardial acid fastness.
作者信息
Beaman B L, Burnside J
出版信息
Appl Microbiol. 1973 Sep;26(3):426-8. doi: 10.1128/am.26.3.426-428.1973.
Abstract
Several strains of Mycobacterium and Nocardia were stained for acid fastness by using the method of Kinyoun. Duplicate, glutaraldehyde-fixed smears were extracted with fresh pyridine prior to staining. The results clearly demonstrated that both in vivo- and in vitro-grown Nocardia differed from Mycobacterium by becoming nonacid fast after extraction. This pyridine extraction of nocardial acid fastness should have diagnostic and taxonomic application.
摘要
使用金扬（Kinyoun）方法对几种分枝杆菌和诺卡氏菌菌株进行抗酸染色。在染色前，用新鲜吡啶提取经戊二醛固定的涂片复制品。结果清楚地表明，体内和体外培养的诺卡氏菌与分枝杆菌不同，提取后变为非抗酸性。这种对诺卡氏菌抗酸性的吡啶提取应具有诊断和分类学应用价值。
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