β-半乳糖苷酶赭石型突变体U118中突变的位置。
Position of the mutation in beta-galactosidase ochre mutant U118.
作者信息
Zabin I, Fowler A V, Beckwith J R
出版信息
J Bacteriol. 1978 Jan;133(1):437-8. doi: 10.1128/jb.133.1.437-438.1978.
Abstract
The Escherichia coli lacZ ochre mutant strain U118 was converted to an amber mutant and suppressed with supF, which inserts tyrosine. Enzymatically active beta-galactosidase was isolated. It contained tyrosine at residue number 17 instead of glutamic acid as in wild type.
摘要
大肠杆菌乳糖操纵子β-半乳糖苷酶赭石突变株U118被转化为琥珀突变体并用supF进行抑制，supF会插入酪氨酸。分离出了具有酶活性的β-半乳糖苷酶。它在第17位残基处含有酪氨酸，而不是野生型中的谷氨酸。
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本文引用的文献
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Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.琥珀抑制：酪氨酸转运RNA反密码子中的核苷酸变化。
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