Fuchs S, Mozes E, Maoz A, Sela M
J Exp Med. 1974 Jan 1;139(1):148-58. doi: 10.1084/jem.139.1.148.
Several inbred mouse strains were screened for their ability to respond to the ordered periodic collagen-like polymer (Pro-Gly-Pro)(n), to the random copolymer (Pro(66), Gly(34))(n), to the protein conjugate Pro-Gly-Pro-ovalbumin, to rat tail tendon collagen, rat tail tendon gelatin, and to Ascaris cuticle collagen. Differences were obtained in the magnitude of the antibody titers towards the above immunogens among the strains tested. The level of the response to the ordered polymer (Pro-Gly-Pro)(n) was not similar to that towards the random (Pro(66), Gly(34))(n), confirming differences in the antigenic determinants of the two immunogens. The role of the thymus in the immune response to (Pro-Gly-Pro)(n) and (Pro(66), Gly(34))(n) as well as to two collagens and gelatin, was studied in order to find out a possible correlation with the structural features of the immunogens. Heavily irradiated recipients were injected with syngeneic thymocytes, marrow cells, or a mixture of both cell populations and were immunized with the above-mentioned antigens. An efficient immune response to the ordered collagen-like (Pro-Gly-Pro)(n) was obtained in the absence of transferred thymocytes. The thymus independence of (Pro-Gly-Pro)(n) was confirmed when thymectomized irradiated mice were used as recipients. In contrast with these results, cooperation between thymus and marrow cells was necessary in order to elicit an immune response to (Pro(56), Gly(34))(n). Similarly, the immune response to the triple helical collagen was found to be independent of the thymus, whereas for an effective response to its denatured product, gelatin, thymus cells were required. These findings indicate that a unique three-dimensional structure of immunogens possessing repeating antigenic determinants plays an important role in determining the need for cell to cell interaction in order to elicit an antibody response.
对几种近交系小鼠进行了筛选，以检测它们对有序周期性胶原样聚合物（Pro-Gly-Pro）(n)、无规共聚物（Pro(66), Gly(34)）(n)、蛋白偶联物Pro-Gly-Pro-卵清蛋白、大鼠尾腱胶原、大鼠尾腱明胶和蛔虫表皮胶原的反应能力。在所测试的品系中，针对上述免疫原的抗体滴度大小存在差异。对有序聚合物（Pro-Gly-Pro）(n)的反应水平与对无规聚合物（Pro(66), Gly(34)）(n)的反应水平不同，这证实了两种免疫原的抗原决定簇存在差异。研究了胸腺在对（Pro-Gly-Pro）(n)和（Pro(66), Gly(34)）(n)以及两种胶原和明胶的免疫反应中的作用，以找出与免疫原结构特征的可能相关性。用同基因胸腺细胞、骨髓细胞或两种细胞群体的混合物注射受重度照射的受体，并使用上述抗原进行免疫。在没有转移胸腺细胞的情况下，对有序胶原样（Pro-Gly-Pro）(n)获得了有效的免疫反应。当将胸腺切除的受照射小鼠用作受体时，证实了（Pro-Gly-Pro）(n)的胸腺非依赖性。与这些结果相反，为了引发对（Pro(56), Gly(34)）(n)的免疫反应，胸腺细胞和骨髓细胞之间的合作是必要的。同样，发现对三螺旋胶原的免疫反应不依赖于胸腺，而对于对其变性产物明胶的有效反应，则需要胸腺细胞。这些发现表明，具有重复抗原决定簇的免疫原的独特三维结构在决定引发抗体反应所需的细胞间相互作用方面起着重要作用。
