Skaer R J, Whytock S
J Cell Sci. 1977;27:13-21. doi: 10.1242/jcs.27.1.13.
The production of an artifactual network in the nuclear sap of the salivary glands of Drosophila has been investigated. Mechanical stress to the cells, 3% glutaraldehyde containing more than 4 mM calcium, tannins, or cacodylate buffer or whose temperature is above 10 degrees C all enhance this artifactual effect. Over the range pH 6.65-7.4 there is no significant effect of pH. The inclusion of sucrose, and adenine nucleotides or other 'chelating' agents in the fixative reduces the effect, especially at low temperature (6-10 degrees C). These influences are additive and can abolish the artifactual effect. So too can 8% glutaraldehyde, but this is irrespective of temperature.
对果蝇唾液腺核液中人工网络的产生进行了研究。对细胞施加机械应力、含有超过4 mM钙的3%戊二醛、单宁酸、二甲胂酸盐缓冲液或温度高于10摄氏度，都会增强这种人工效应。在pH 6.65 - 7.4范围内，pH值没有显著影响。在固定剂中加入蔗糖、腺嘌呤核苷酸或其他“螯合剂”可降低这种效应，尤其是在低温（6 - 10摄氏度）下。这些影响是相加的，并且可以消除人工效应。8%的戊二醛也可以消除这种效应，但这与温度无关。
