Skaer R J, Whytock S
J Cell Sci. 1977 Aug;26:301-10. doi: 10.1242/jcs.26.1.301.
Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm in diameter - the size of nucleosomes - and are interconnected by exceedingly fine fibrils. Each fixative and buffer imposes its own distinctive, reproducible pattern on nuclear sap. The structure of nuclear sap in life cannot be deduced from this range of patterns.
核液在戊二醛中固定后会形成一个纤维网络，其尺寸和染色特性与染色质相似。在细胞核切片中，这种人为形成的网络很容易与真正的染色质纤维混淆。用甲醛处理会产生均匀排列的珠子。这些珠子直径约为10纳米——即核小体的大小——并由极其纤细的纤维相互连接。每种固定剂和缓冲液都会在核液上形成其独特的、可重复的模式。无法从这一系列模式中推断出核液在活细胞中的结构。
