• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外细胞内感染进程的定量评估：麻风分枝杆菌在培养的血液单核细胞中掺入³H-胸腺嘧啶核苷至脱氧核糖核酸中。

Quantitative evaluation of the progress of intracellular infection in vitro: incorporation of 3H-thymidine into deoxyribonucleic acid by Mycobacterium leprae in cultivated blood monocytes.

作者信息

Talwar G P, Krishnan A D, Gupta P D

出版信息

Infect Immun. 1974 Jan;9(1):187-91. doi: 10.1128/iai.9.1.187-191.1974.

DOI:10.1128/iai.9.1.187-191.1974
PMID:4128631
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC414783/
Abstract

Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-(3)H]thymidine into deoxyribonucleic acid of the bacterial cells. The radioactive precursor is not taken up by the host cells, and evidence has been presented for its incorporation into bacteria. The procedure is sensitive, reproducible, and highly quantitative.

摘要

诸如麻风分枝杆菌等细胞内寄生虫在源自人外周血单核细胞的巨噬细胞中的生长情况，可通过将[甲基 -（3）H]胸腺嘧啶核苷选择性掺入细菌细胞的脱氧核糖核酸来评估。放射性前体不会被宿主细胞摄取，并且已有证据表明其掺入了细菌中。该方法灵敏、可重复且高度定量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/45211d04bdc6/iai00241-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/54ad7f061695/iai00241-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/a2cc563e9792/iai00241-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/45211d04bdc6/iai00241-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/54ad7f061695/iai00241-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/a2cc563e9792/iai00241-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0de/414783/45211d04bdc6/iai00241-0203-a.jpg

相似文献

1
Quantitative evaluation of the progress of intracellular infection in vitro: incorporation of 3H-thymidine into deoxyribonucleic acid by Mycobacterium leprae in cultivated blood monocytes.体外细胞内感染进程的定量评估：麻风分枝杆菌在培养的血液单核细胞中掺入³H-胸腺嘧啶核苷至脱氧核糖核酸中。
Infect Immun. 1974 Jan;9(1):187-91. doi: 10.1128/iai.9.1.187-191.1974.
2
Incorporation of 3H-thymidine in Mycobacterium leprae within differentiated human macrophages.3H-胸腺嘧啶核苷在分化的人类巨噬细胞内掺入麻风分枝杆菌的情况。
J Med Microbiol. 1981 Aug;14(3):279-93. doi: 10.1099/00222615-14-3-279.
3
Incorporation of tritiated thymidine by leprosy bacilli in cultures of human lepromatous macrophages.
J Infect Dis. 1972 Apr;125(4):416-9. doi: 10.1093/infdis/125.4.416.
4
Lack of correlation between morphological index and viability as assessed by the uptake of 3H-thymidine by macrophage resident M. leprae.
Lepr India. 1982 Jul;54(3):420-7.
5
Progress in the radiolabeling of Mycobacterium leprae.麻风分枝杆菌放射性标记的进展。
Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):65-71.
6
Primary dapsone resistance as assessed by uptake of labelled thymidine by the macrophage resident Mycobacterium leprae.
Indian J Lepr. 1989 Oct;61(4):437-41.
7
The uptake of 3H-thymidine in Mycobacterium leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability. Factors influencing the specificity of the in vitro assay.
Int J Lepr Other Mycobact Dis. 1981 Jun;49(2):187-93.
8
Comparison of radiometric macrophage assay and fluorescein diacetate/ethidium bromide staining for evaluation of M. leprae viability.用于评估麻风分枝杆菌活力的放射测量巨噬细胞测定法与荧光素二乙酸酯/溴化乙锭染色法的比较。
Int J Lepr Other Mycobact Dis. 1987 Jun;55(2):316-21.
9
Uptake of radioactive DOPA by M. leprae.麻风杆菌对放射性多巴的摄取
Nature. 1974 Jun 28;249(460):854-5. doi: 10.1038/249854a0.
10
Mycobacterium leprae upregulates IRGM expression in monocytes and monocyte-derived macrophages.麻风分枝杆菌在单核细胞和单核细胞衍生的巨噬细胞中上调 IRGM 表达。
Inflammation. 2014 Aug;37(4):1028-34. doi: 10.1007/s10753-014-9825-1.

引用本文的文献

1
A destiny to fulfill.有待实现的命运。
J Biosci. 2005 Sep;30(4):435-47. doi: 10.1007/BF02703716.
2
Rapid, radiolabeled macrophage culture method for detection of dapsone-resistant Mycobacterium leprae.用于检测耐氨苯砜麻风分枝杆菌的快速放射性标记巨噬细胞培养方法。
Antimicrob Agents Chemother. 1982 Jan;21(1):26-32. doi: 10.1128/AAC.21.1.26.
3
Preliminary demonstration of human tuberculoimmunity in vitro.人体结核免疫体外初步论证

本文引用的文献

1
A GUIDE TO THE COUNTING OF MYCOBACTERIA IN CLINICAL AND EXPERIMENTAL MATERIALS.临床和实验材料中分枝杆菌计数指南
Int J Lepr. 1964 Apr-Jun;32:156-67.
2
Improvements in epoxy resin embedding methods.环氧树脂包埋方法的改进。
J Biophys Biochem Cytol. 1961 Feb;9(2):409-14. doi: 10.1083/jcb.9.2.409.
3
Incorporation of tritiated thymidine by leprosy bacilli in cultures of human lepromatous macrophages.
J Infect Dis. 1972 Apr;125(4):416-9. doi: 10.1093/infdis/125.4.416.
Infect Immun. 1981 Jan;31(1):453-64. doi: 10.1128/iai.31.1.453-464.1981.
4
Pathology and pathogenesis of predilective sites of nerve damage in leprous neuritis. Nerves in the arm and the face.麻风性神经炎中神经损伤好发部位的病理学与发病机制。手臂和面部的神经。
Neurosurg Rev. 1983;6(3):139-52. doi: 10.1007/BF01742765.
5
Biological, chemical, immunological and staining properties of bacteria isolated from tissues of leprosy patients.从麻风病患者组织中分离出的细菌的生物学、化学、免疫学及染色特性。
Eur J Epidemiol. 1985 Sep;1(3):202-31. doi: 10.1007/BF00234095.