Huang Chunzi, Zhao Zengjing, Wu Xing, Zhao Hu, Wang Meng, He Zhi, Li Zongjun, Wang Lihao, Tang Yafei, Wang Risheng, He Longfei, Gong Mingxia
Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Guangxi Key Laboratory of Vegetable Breeding and New Technology Development, Nanning 530007, China.
Viruses. 2025 Oct 31;17(11):1456. doi: 10.3390/v17111456.
(ChiVMV) causes severe yield losses in pepper across Asia. It is very urgent to study the host plant resistance to control this viral disease. As a type of defense response gene, pathogenesis-related protein 1 (PR1) is a well-established defense marker against fungal/bacterial pathogens, and its role in virus resistance remains unclear. Here, we cloned from the ChiVMV-highly resistant pepper variety 'Perennial'. The 477 bp ORF encodes a 17.65 kDa basic protein containing a conserved CAP-PR1 domain. The subcellular localization of revealed that it was located in the plasma membrane, endoplasmic reticulum (ER), and nucleus. RT-qPCR revealed leaf-predominant expression, with earlier and stronger induction in the highly resistant than the highly susceptible variety after ChiVMV inoculation (6.4-fold at 2 days post-inoculation). The overexpression of in tobacco significantly increased resistance, reducing disease index by 25% and viral coat protein accumulation. Our findings identified as a positive regulator of ChiVMV resistance, providing a molecular target for pepper breeding. In addition, exogenous SA treatment increased the resistance of the highly susceptible cultivar 'Guijiao 12' to ChiVMV, and 0.25 mM had a greater effect.
辣椒脉斑驳病毒（ChiVMV）在亚洲各地导致辣椒严重减产。研究寄主植物抗性以控制这种病毒性疾病迫在眉睫。作为一种防御反应基因，病程相关蛋白1（PR1）是一种针对真菌/细菌病原体的成熟防御标记，其在病毒抗性中的作用仍不清楚。在此，我们从对ChiVMV高度抗性的辣椒品种‘Perennial’中克隆了（该基因）。477 bp的开放阅读框编码一个17.65 kDa的碱性蛋白，含有一个保守的CAP-PR1结构域。（该基因的）亚细胞定位显示其位于质膜、内质网（ER）和细胞核中。逆转录定量PCR显示其在叶片中优势表达，在接种ChiVMV后，高抗品种比高感品种诱导更早且更强（接种后2天为6.4倍）。（该基因）在烟草中的过表达显著提高了抗性，病害指数降低了25%，病毒外壳蛋白积累减少。我们的研究结果确定（该基因）为ChiVMV抗性的正调控因子，为辣椒育种提供了一个分子靶点。此外，外源水杨酸处理提高了高感品种‘桂椒12’对ChiVMV的抗性，0.25 mM的效果更佳。
