AlHudaib Khalid A, Alanazi Naimah Asid, Ghorbel Mouna, El-Ganainy Sherif Mohamed, Brini Faiçal
Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia.
Pests and Plant Diseases Unit, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia.
Plants (Basel). 2022 Aug 31;11(17):2284. doi: 10.3390/plants11172284.
Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat ( L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from PRB1-2-like, PRB1-2-like, and subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat.
病程相关蛋白1（PR-1）在调节植物对生物和非生物胁迫的反应中起关键作用。本研究旨在分离和鉴定燕麦（ ）中的首个PR-1基因（AvPR-1）。AvPR-1在功能蛋白结构域中呈现出保守的信号肽基序和核心氨基酸组成，因为AvPR-1的蛋白质序列与从PRB1-2-like、PRB1-2-like和节节麦亚种中分离出的已知PR1蛋白分别具有98.28%、97.7%和95.4%的同一性。生物信息学分析表明，AvPR-1蛋白属于CAP超家族（PF00188）。还对AvPR-1蛋白进行了二级和三维结构分析，证实了PR-1在所研究物种中的序列保守性。如先前对其小麦同源物TdPR1.2的研究所示，AvPR-1蛋白在其C末端部分含有一个钙调蛋白结合结构域。此外，基因表达分析表明，AvPR-1在响应多种非生物和激素胁迫时被诱导，尤其是在处理48小时后的叶片中。这是第一项展示燕麦中AvPR-1基因在不同胁迫下表达谱的研究。
