用于研究髓鞘的魏格特染色切片与未固定、未染色切片的比较。
Comparison of Weigert stained sections with unfixed, unstained sections for study of myelin sheaths.
作者信息
Richter C P, Warner C L
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):598-601. doi: 10.1073/pnas.71.3.598.
Abstract
Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the animal and immediately photographed for permanent records. Such sections have value for neuroanatomical studies, and for rapid localization of various types of experimental lesions.
摘要
在刚处死的未注射动物的薄的未染色脑切片中可以显示出有髓纤维。用这种方法，有髓纤维像在福尔马林固定脑的魏格特染色切片中一样清晰突出。未固定的未染色切片在动物死后几分钟内就可以在普通光线下观察，并立即拍照以作永久记录。这样的切片对神经解剖学研究以及各种类型实验性损伤的快速定位有价值。
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