RNA 指导的 DNA 聚合酶对小鼠肉瘤 - 白血病病毒的转录程度。
Extent of transcription of mouse sarcoma-leukemia virus by RNA-directed DNA polymerase.
作者信息
Tavitian A, Hamelin R, Tchen P, Olofsson B, Boiron M
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):755-9. doi: 10.1073/pnas.71.3.755.
The DNA product obtained from the endogenous RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) reaction of the Moloney sarcoma:leukemia viruses produced by the 78 A-1 cell line was analyzed and characterized. The extent of transcription of viral 70S RNA was measured by RNA.DNA hybridization ((32)P-viral RNA-(3)H product DNA). No double-stranded DNA was obtained. The product consisted of 95-99% single-stranded DNA with an average length of 200 nucleotides. In contrast to the results reported with avian and other RNA oncogenic viruses, it was found that the entire 70S viral RNA genome was transcribed into DNA pieces and that a small excess of the product DNA was sufficient to anneal the 70S RNA and render it totally resistant to single-stranded-specific enzyme digestion.
对由78 A - 1细胞系产生的莫洛尼肉瘤 - 白血病病毒的内源性RNA指导的DNA聚合酶（脱氧核苷三磷酸：DNA脱氧核苷酸转移酶，EC 2.7.7.7）反应所获得的DNA产物进行了分析和表征。通过RNA - DNA杂交（（32）P - 病毒RNA - （3）H产物DNA）测定病毒70S RNA的转录程度。未获得双链DNA。该产物由95 - 99％的单链DNA组成，平均长度为200个核苷酸。与关于禽和其他RNA致癌病毒所报道的结果相反，发现整个70S病毒RNA基因组被转录成DNA片段，并且少量过量的产物DNA足以使70S RNA退火并使其完全抵抗单链特异性酶消化。
Zotero 插件