RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。
RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.
作者信息
Leis J P, Hurwitz J
出版信息
J Virol. 1972 Jan;9(1):130-42. doi: 10.1128/JVI.9.1.130-142.1972.
Abstract
The role of ribonucleic acid (RNA) in deoxyribonucleic acid (DNA) synthesis with the purified DNA polymerase from the avian myeloblastosis virus has been studied. The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg(2+), and a variety of RNA templates including those isolated from avian myeloblastosis, Rous sarcoma, and Rauscher leukemia viruses; phages f2, MS2, and Qbeta; and synthetic homopolymers such as polyadenylate.polyuridylic acid. The enzyme does not initiate the synthesis of new chains but incorporates deoxynucleotides at 3' hydroxyl ends of primer strands. The product is an RNA.DNA hybrid in which the two polynucleotide components are covalently linked. Free DNA has not been detected among the products formed with the purified enzyme in vitro. The DNA synthesized with avian myeloblastosis virus RNA after alkaline hydrolysis has a sedimentation coefficient of 6 to 7S.
摘要
人们已经研究了核糖核酸（RNA）在利用禽成髓细胞瘤病毒纯化的DNA聚合酶进行脱氧核糖核酸（DNA）合成中的作用。该聚合酶在四种脱氧核苷三磷酸、Mg(2+)以及多种RNA模板存在的情况下催化DNA合成，这些RNA模板包括从禽成髓细胞瘤、劳氏肉瘤和劳斯氏白血病病毒中分离得到的模板；噬菌体f2、MS2和Qβ；以及合成的同聚物，如聚腺苷酸-聚尿苷酸。该酶不会起始新链的合成，而是在引物链的3'羟基末端掺入脱氧核苷酸。产物是一种RNA-DNA杂交体，其中两个多核苷酸组分通过共价键相连。在体外利用纯化的酶形成的产物中未检测到游离的DNA。用禽成髓细胞瘤病毒RNA合成的DNA经碱性水解后沉降系数为6至7S。
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