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1
RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。
J Virol. 1972 Jan;9(1):130-42. doi: 10.1128/JVI.9.1.130-142.1972.
2
RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。I. 反应中DNA的直接影响。
J Virol. 1972 Jan;9(1):116-29. doi: 10.1128/JVI.9.1.116-129.1972.
3
Avian myeloblastosis virus DNA polymerase: initiation of DNA synthesis and an associated ribonuclease.禽成髓细胞瘤病毒DNA聚合酶：DNA合成的起始及一种相关的核糖核酸酶
Johns Hopkins Med J Suppl. 1973;2:193-8.
4
RNA-DNA covalent bonds between the RNA primers and the DNA products formed by RNA tumor virus DNA polymerase.RNA肿瘤病毒DNA聚合酶形成的RNA引物与DNA产物之间的RNA-DNA共价键。
J Virol. 1973 Dec;12(6):1491-502. doi: 10.1128/JVI.12.6.1491-1502.1973.
5
Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase.60至70S禽肿瘤病毒核糖核酸亚基在其作为病毒脱氧核糖核酸聚合酶模板活性中的作用。
J Virol. 1972 Jul;10(1):23-31. doi: 10.1128/JVI.10.1.23-31.1972.
6
RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.五种RNA病毒中的RNA依赖性DNA聚合酶活性：二价阳离子需求
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96. doi: 10.1073/pnas.67.4.1789.
7
Deoxyribonucleic acid polymerase associated with Rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product.与劳氏肉瘤病毒和禽成髓细胞瘤病毒相关的脱氧核糖核酸聚合酶：酶及其产物的特性
J Virol. 1970 Nov;6(5):589-98. doi: 10.1128/JVI.6.5.589-598.1970.
8
Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses.致癌RNA病毒中DNA指导的DNA聚合酶产物的特性分析
Nature. 1970 Aug 8;227(5258):563-7. doi: 10.1038/227563a0.
9
The inhibition of avian myeloblastosis virus deoxyribonucleic acid polymerase by synthetic polynucleotides.合成多核苷酸对禽成髓细胞瘤病毒脱氧核糖核酸聚合酶的抑制作用。
Biochemistry. 1974 Apr 23;13(9):1987-93. doi: 10.1021/bi00706a032.
10
DNA-directed DNA polymerase activity in oncogenic RNA viruses.致癌RNA病毒中的DNA指导的DNA聚合酶活性。
Nature. 1970 Sep 5;227(5262):1029-31. doi: 10.1038/2271029a0.

引用本文的文献

1
Mechanism analysis indicates that recombination events in HIV-1 initiate and complete over short distances, explaining why recombination frequencies are similar in different sections of the genome.机制分析表明，HIV-1中的重组事件在短距离内启动并完成，这解释了为什么基因组不同区域的重组频率相似。
J Mol Biol. 2009 Apr 24;388(1):30-47. doi: 10.1016/j.jmb.2009.02.029. Epub 2009 Feb 20.
2
Covalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase.禽成髓细胞瘤病毒脱氧核糖核酸聚合酶的核糖核酸引物与脱氧核糖核酸产物之间的共价连接。
J Virol. 1972 Oct;10(4):622-7. doi: 10.1128/JVI.10.4.622-627.1972.
3
Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids.位于67B的四个果蝇热休克基因：重组质粒的特性分析
Nucleic Acids Res. 1980 Oct 10;8(19):4441-57. doi: 10.1093/nar/8.19.4441.
4
Extent of double strandedness in avian myeloblastosis virus RNA.禽成髓细胞瘤病毒RNA的双链化程度
J Virol. 1974 Dec;14(6):1458-62. doi: 10.1128/JVI.14.6.1458-1462.1974.
5
Strandedness and complementarity of DNA from long-term RNA-dependent DNA polymerase reactions of Soehner-Dmochowski murine sarcoma virus.来自索纳-德莫乔夫斯基小鼠肉瘤病毒长期RNA依赖性DNA聚合酶反应的DNA的链状结构和互补性。
J Virol. 1973 Nov;12(5):1049-64. doi: 10.1128/JVI.12.5.1049-1064.1973.
6
RNA-DNA covalent bonds between the RNA primers and the DNA products formed by RNA tumor virus DNA polymerase.RNA肿瘤病毒DNA聚合酶形成的RNA引物与DNA产物之间的RNA-DNA共价键。
J Virol. 1973 Dec;12(6):1491-502. doi: 10.1128/JVI.12.6.1491-1502.1973.
7
Structural rearrangement and subunit composition of RNA from released Soehner-Dmochowski murine sarcoma virions.释放的索纳-德莫乔夫斯基小鼠肉瘤病毒粒子中RNA的结构重排与亚基组成
J Virol. 1973 May;11(5):709-20. doi: 10.1128/JVI.11.5.709-720.1973.
8
Isolation and characterization of a protein that stimulates DNA synthesis from avian myeloblastosis virus.从禽成髓细胞瘤病毒中分离并鉴定一种刺激DNA合成的蛋白质。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2331-5. doi: 10.1073/pnas.69.8.2331.
9
Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase.60至70S禽肿瘤病毒核糖核酸亚基在其作为病毒脱氧核糖核酸聚合酶模板活性中的作用。
J Virol. 1972 Jul;10(1):23-31. doi: 10.1128/JVI.10.1.23-31.1972.
10
RNA-primed DNA synthesis in vitro.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1560-4. doi: 10.1073/pnas.69.6.1560.

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Allosteric proteins and cellular control systems.别构蛋白与细胞控制系统。
J Mol Biol. 1963 Apr;6:306-29. doi: 10.1016/s0022-2836(63)80091-1.
2
Specific inhibition by ATP and other properites of an endonuclease of Neurospora crassa.粗糙脉孢菌一种核酸内切酶受ATP的特异性抑制及其它特性
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Forms of deoxyribonucleic acid produced by virions of the ribonucleic acid tumor viruses.由核糖核酸肿瘤病毒的病毒粒子产生的脱氧核糖核酸形式。
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T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.T4噬菌体基因32：DNA复制与重组中的一种结构蛋白。
Nature. 1970 Sep 26;227(5265):1313-8. doi: 10.1038/2271313a0.
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Formation of viral RNA-DNA hybrid molecules by the DNA polymerase of sarcoma-leukaemia viruses.肉瘤白血病病毒的DNA聚合酶形成病毒RNA-DNA杂交分子。
Nature. 1970 Sep 5;227(5262):1026-8. doi: 10.1038/2271026a0.
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The RNA of influenza virus.流感病毒的核糖核酸
Proc Natl Acad Sci U S A. 1968 Mar;59(3):930-7. doi: 10.1073/pnas.59.3.930.
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Nucleic acid and proteins isolated from the Rauscher mouse leukemia virus (MLV).从劳氏鼠白血病病毒（MLV）中分离出的核酸和蛋白质。
Proc Natl Acad Sci U S A. 1966 Jan;55(1):219-27. doi: 10.1073/pnas.55.1.219.
8
Mung bean nuclease I. II. Resistance of double stranded deoxyribonucleic acid and susceptibility of regions rich in adenosine and thymidine to enzymatic hydrolysis.绿豆核酸酶I。II。双链脱氧核糖核酸的抗性以及富含腺苷和胸腺嘧啶区域对酶促水解的敏感性。
J Biol Chem. 1970 Feb 25;245(4):891-8.
9
RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。I. 反应中DNA的直接影响。
J Virol. 1972 Jan;9(1):116-29. doi: 10.1128/JVI.9.1.116-129.1972.
10
Deoxyribonucleic acid polymerase of Rous sarcoma virus: studies on the mechanism of double-stranded deoxyribonucleic acid synthesis.劳氏肉瘤病毒的脱氧核糖核酸聚合酶：双链脱氧核糖核酸合成机制的研究
J Virol. 1971 May;7(5):539-48. doi: 10.1128/JVI.7.5.539-548.1971.

RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。

RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.

作者信息

Leis J P, Hurwitz J

出版信息

J Virol. 1972 Jan;9(1):130-42. doi: 10.1128/JVI.9.1.130-142.1972.

DOI:10.1128/JVI.9.1.130-142.1972
PMID:4333539
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356271/
Abstract

The role of ribonucleic acid (RNA) in deoxyribonucleic acid (DNA) synthesis with the purified DNA polymerase from the avian myeloblastosis virus has been studied. The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg(2+), and a variety of RNA templates including those isolated from avian myeloblastosis, Rous sarcoma, and Rauscher leukemia viruses; phages f2, MS2, and Qbeta; and synthetic homopolymers such as polyadenylate.polyuridylic acid. The enzyme does not initiate the synthesis of new chains but incorporates deoxynucleotides at 3' hydroxyl ends of primer strands. The product is an RNA.DNA hybrid in which the two polynucleotide components are covalently linked. Free DNA has not been detected among the products formed with the purified enzyme in vitro. The DNA synthesized with avian myeloblastosis virus RNA after alkaline hydrolysis has a sedimentation coefficient of 6 to 7S.

摘要

人们已经研究了核糖核酸（RNA）在利用禽成髓细胞瘤病毒纯化的DNA聚合酶进行脱氧核糖核酸（DNA）合成中的作用。该聚合酶在四种脱氧核苷三磷酸、Mg(2+)以及多种RNA模板存在的情况下催化DNA合成，这些RNA模板包括从禽成髓细胞瘤、劳氏肉瘤和劳斯氏白血病病毒中分离得到的模板；噬菌体f2、MS2和Qβ；以及合成的同聚物，如聚腺苷酸-聚尿苷酸。该酶不会起始新链的合成，而是在引物链的3'羟基末端掺入脱氧核苷酸。产物是一种RNA-DNA杂交体，其中两个多核苷酸组分通过共价键相连。在体外利用纯化的酶形成的产物中未检测到游离的DNA。用禽成髓细胞瘤病毒RNA合成的DNA经碱性水解后沉降系数为6至7S。