皮钦德病毒的聚合酶活性
Polymerase activity of Pichinde virus.
作者信息
Carter M F, Biswal N, Rawls W E
出版信息
J Virol. 1974 Mar;13(3):577-83. doi: 10.1128/JVI.13.3.577-583.1974.
Abstract
Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg(2+) and Mn(2+) were required for optimal reactivity. The RNA product was partially resistant to RNase and the resistant portion had a sedimentation coefficient of 22 to 26S in sucrose gradients.
摘要
对沙粒病毒科成员之一的皮钦德病毒进行了聚合酶活性检测。发现纯化的病毒含有依赖RNA的RNA聚合酶，但不具有依赖RNA的DNA聚合酶活性。由于核糖核酸酶（RNase）能抑制内源性聚合酶反应，而脱氧核糖核酸酶（DNase）和放线菌素D均不能，因此病毒的RNA似乎被用作模板。二价阳离子镁离子（Mg2+）和锰离子（Mn2+）是实现最佳反应活性所必需的。RNA产物对RNase有部分抗性，在蔗糖梯度中，抗性部分的沉降系数为22至26S。
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