水泡性口炎病毒的病毒粒子相关RNA聚合酶在体外合成含聚腺苷酸的RNA。
In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus.
作者信息
Banerjee A K, Rhodes D P
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3566-70. doi: 10.1073/pnas.70.12.3566.
Abstract
The RNA synthesized in vitro by the virion-associated RNA-instructed RNA polymerase of purified vesicular stomatitis virus contains polyadenylate sequences. These have been demonstrated by their partial resistance to pancreatic and T(1) ribonucleases and their capacity to bind to poly(U) filters and oligo(dT)-cellulose. The polyadenylate sequences range in apparent size from 50 to 200 bases, similar to the size of the poly(A) in mRNA from vesicular stomatitis virus-infected cells. Possible mechanisms of polyadenylylation of the in vitro RNA product are discussed.
摘要
由纯化的水疱性口炎病毒的病毒体相关的RNA指导的RNA聚合酶在体外合成的RNA含有聚腺苷酸序列。这些序列已通过它们对胰核糖核酸酶和T(1)核糖核酸酶的部分抗性以及它们与聚(U)滤膜和寡聚(dT)-纤维素结合的能力得到证实。聚腺苷酸序列的表观大小范围为50至200个碱基，类似于水疱性口炎病毒感染细胞的mRNA中的聚(A)大小。文中讨论了体外RNA产物聚腺苷酸化的可能机制。
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