皮钦德病毒RNA的滞留
Strandedness of Pichinde virus RNA.
作者信息
Leung W C, Ghosh H P, Rawls W E
出版信息
J Virol. 1977 Apr;22(1):235-7. doi: 10.1128/JVI.22.1.235-237.1977.
Abstract
The Pichinde virus RNA did not possess the following characteristics of eucaryotic mRNA: polyadenylic acid sequence, capped methylated structure, and ability to direct protein synthesis in vitro. Polysomal RNA extracted from cells infected with Pichinde virus reannealed with 32P-labeled virus RNA, protecting about 60% of the latter against RNase degestion. The polyadenylic acid-containing polysomal RNA also reannealed to the 32P-labeled virus RNA to approximately the same extent. These indicate that the major part of the genomic RNA of Pichinde virus is negative stranded.
摘要
皮钦德病毒RNA不具备真核生物信使核糖核酸的以下特征：聚腺苷酸序列、加帽甲基化结构以及体外指导蛋白质合成的能力。从感染皮钦德病毒的细胞中提取的多核糖体RNA与32P标记的病毒RNA重新退火，保护后者约60%不被核糖核酸酶消化。含聚腺苷酸的多核糖体RNA与32P标记的病毒RNA重新退火的程度大致相同。这些表明皮钦德病毒基因组RNA的主要部分是负链的。
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