Wolff K L, Muth D J, Hudson B W, Trent D W
J Clin Microbiol. 1981 Aug;14(2):135-40. doi: 10.1128/jcm.14.2.135-140.1981.
Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and flavivirus antigens were tested by both the SPRIA procedure and conventional serological methods. Standards were established for use of the crude antigens in the SPRIA procedure. Reproducibility of the procedure was similar to that of conventional tests. The SPRIA procedure specifically differentiated SLE from clinically similar alphaviruses, but did not differentiate SLE from related flaviviruses very well. SPRIA diagnosis of SLE infections by the immunoglobulin G response in paired sera was good. Generally, the crude antigen SPRIA procedure was as sensitive as the conventional serological tests, but not as specific.
由于在固相放射免疫分析（SPRIA）程序中使用高度纯化的病毒蛋白对于常规诊断血清学而言并不实用，因此该程序被调整为适用于目前常用于诊断圣路易斯脑炎（SLE）病毒感染的粗制抗原。通过SPRIA程序和传统血清学方法对SLE以及各种其他甲型和黄病毒抗原进行了检测。建立了在SPRIA程序中使用粗制抗原的标准。该程序的可重复性与传统测试相似。SPRIA程序能够将SLE与临床症状相似的甲型病毒特异性区分开来，但在区分SLE与相关黄病毒方面效果不佳。通过检测配对血清中的免疫球蛋白G反应，SPRIA对SLE感染的诊断效果良好。总体而言，粗制抗原SPRIA程序与传统血清学测试一样灵敏，但特异性不如传统测试。
