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用免疫扩散技术分析阴道棒状杆菌。

Analysis of Corynebacterium vaginale by an immunodiffusion technique.

作者信息

Smaron M F, Vice J L

出版信息

Appl Microbiol. 1974 Mar;27(3):469-74. doi: 10.1128/am.27.3.469-474.1974.

DOI:10.1128/am.27.3.469-474.1974
PMID:4132956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380067/
Abstract

An immunodiffusion technique was employed to study the antigenic relationship of Corynebacterium vaginale isolates, vaginal diphtheroids, and members of the genera Corynebacterium and Lactobacillus. Antisera were prepared against C. vaginale ATCC strain 14018 grown diphasically and on blood agar plates and were tested against extracts of organisms prepared by sonication. Ouchterlony analysis demonstrated that all of the isolates of C. vaginale examined possess a common antigenic determinant. No antigenic relationship was detected between C. vaginale and members of the genera Corynebacterium or Lactobacillus. This study also demonstrated that alterations in the cultural conditions can cause variations in the antigenic composition of C. vaginale.

摘要

采用免疫扩散技术研究阴道棒状杆菌分离株、阴道类白喉杆菌以及棒状杆菌属和乳杆菌属成员之间的抗原关系。制备了针对在双相培养基和血琼脂平板上生长的阴道棒状杆菌ATCC 14018菌株的抗血清,并对经超声处理制备的生物体提取物进行了检测。双向免疫扩散分析表明,所有检测的阴道棒状杆菌分离株都具有共同的抗原决定簇。未检测到阴道棒状杆菌与棒状杆菌属或乳杆菌属成员之间的抗原关系。本研究还表明,培养条件的改变可导致阴道棒状杆菌抗原组成的变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/a33f9e70dd77/applmicro00038-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/23b3bd0fda0d/applmicro00038-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/673cf7f6c1f0/applmicro00038-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/8bc188233a30/applmicro00038-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/a33f9e70dd77/applmicro00038-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/23b3bd0fda0d/applmicro00038-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/673cf7f6c1f0/applmicro00038-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/8bc188233a30/applmicro00038-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9b2/380067/a33f9e70dd77/applmicro00038-0058-b.jpg

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1
Analysis of Corynebacterium vaginale by an immunodiffusion technique.用免疫扩散技术分析阴道棒状杆菌。
Appl Microbiol. 1974 Mar;27(3):469-74. doi: 10.1128/am.27.3.469-474.1974.
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J Clin Microbiol. 1989 Sep;27(9):2138-9. doi: 10.1128/jcm.27.9.2138-2139.1989.
2
Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale.阴道棒状杆菌细胞外抗原的免疫学和化学特性
Infect Immun. 1977 Nov;18(2):356-62. doi: 10.1128/iai.18.2.356-362.1977.

本文引用的文献

1
IMMUNE DIFFUSION ANALYSIS OF THE EXTRACELLULAR SOLUBLE ANTIGENS OF TWO STRAINS OF RHIZOBIUM MELILOTI.两株苜蓿根瘤菌胞外可溶性抗原的免疫扩散分析
J Bacteriol. 1964 Sep;88(3):782-94. doi: 10.1128/jb.88.3.782-794.1964.
2
Growth requirements of Haemophilus vaginalis.阴道嗜血杆菌的生长需求。
Antonie Van Leeuwenhoek. 1969;35(2):129-45. doi: 10.1007/BF02219124.
3
Method for isolation and identification of Corynebacterium vaginale (Haemophilus vaginalis).阴道棒状杆菌(阴道嗜血杆菌)的分离与鉴定方法。
Appl Microbiol. 1970 Jan;19(1):47-52. doi: 10.1128/am.19.1.47-52.1970.