Smaron M F, Vice J L
Infect Immun. 1977 Nov;18(2):356-62. doi: 10.1128/iai.18.2.356-362.1977.
Supernatants from 72-h peptone-starch-dextrose broth cultures of Corynebacterium vaginale contained significant quantities of three extracellular, soluble antigens (ESA). The ESA were concentrated and partially purified by dialysis followed by ammonium sulfate and ethanol precipitation. Diethylaminoethylcellulose columns were used to isolate two of the three ESA. The ESA were shown to be similar to antigens found on whole C. vaginale cells. Absorption studies indicated that the cell antigens are located at, or near, the surface. On the bases of heat stability, resistance to protease treatment, concanavalin A binding activity, and susceptibility to periodate, it appears that all three ESA are polysaccharide or glycoprotein in nature.
阴道棒状杆菌72小时蛋白胨 - 淀粉 - 葡萄糖肉汤培养物的上清液中含有大量三种细胞外可溶性抗原（ESA）。通过透析，然后硫酸铵和乙醇沉淀，对ESA进行浓缩和部分纯化。使用二乙氨基乙基纤维素柱分离三种ESA中的两种。结果表明，ESA与在完整阴道棒状杆菌细胞上发现的抗原相似。吸收研究表明，细胞抗原位于表面或表面附近。基于热稳定性、对蛋白酶处理的抗性、伴刀豆球蛋白A结合活性以及对高碘酸盐的敏感性，似乎所有三种ESA本质上都是多糖或糖蛋白。
