伊文思蓝作为复染剂，用于重症肌无力患者肌肉抗体免疫荧光检测。
Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis.
作者信息
Closs O, Aarli J A
出版信息
J Clin Pathol. 1974 Feb;27(2):162-7. doi: 10.1136/jcp.27.2.162.
Abstract
The binding of muscle antibodies to skeletal muscle was studied by immunofluorescence techniques. The non-specific fluorescence was not significantly reduced by absorption of the conjugate by tissue powder but was abolished by counterstaining with a 0.1% Evans blue. Possible explanations for this effect are discussed. By this method, immunofluorescence may become more useful in the demonstration of muscle antibodies.
摘要
采用免疫荧光技术研究了肌肉抗体与骨骼肌的结合情况。用组织粉末吸收结合物后，非特异性荧光没有显著降低，但用0.1%伊文思蓝复染可消除非特异性荧光。文中讨论了对此效应的可能解释。通过这种方法，免疫荧光在肌肉抗体的显示方面可能会更有用。
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