Romano N, Scarlata G
Infect Immun. 1974 Jun;9(6):1062-5. doi: 10.1128/iai.9.6.1062-1065.1974.
Complement-fixing activity of a T strain of Mycoplasma was found to be associated with its lipid components. Heat stability and lability of periodate and beta-glucosidase treatments led to the conclusion that complement-fixing, active lipids had carboydrate determinants. Periodate treatment of chromatographic fractions of a chloroform-methanol extract showed that only antigens contained in the acetone fraction were periodate labile. Lipids also appeared to be involved in the passive hemagglutination, since the lipid fraction active in complement fixation also combined and blocked the action of antibodies which agglutinated sensitized erythrocytes with strain P108.
发现一株支原体T株的补体结合活性与其脂质成分有关。高碘酸盐和β-葡萄糖苷酶处理的热稳定性和不稳定性得出结论，具有补体结合活性的脂质具有碳水化合物决定簇。对氯仿-甲醇提取物的色谱馏分进行高碘酸盐处理表明，只有丙酮馏分中的抗原对高碘酸盐不稳定。脂质似乎也参与了被动血凝反应，因为在补体结合中具有活性的脂质馏分也能结合并阻断用P108株致敏红细胞的抗体的作用。
